Global Emergence of Resistance to Fluconazole and Voriconazole in Candida parapsilosis in Tertiary Hospitals in Spain During the COVID-19 Pandemic.

Background: Candida parapsilosis is a frequent cause of candidemia worldwide. Its incidence is associated with the use of medical implants, such as central venous catheters or parenteral nutrition. This species has reduced susceptibility to echinocandins, and it is susceptible to polyenes and azoles. Multiple outbreaks caused by fluconazole-nonsusceptible strains have been reported recently. A similar trend has been observed among the C. parapsilosis isolates received in the last 2 years at the Spanish Mycology Reference Laboratory. Methods: Yeast were identified by molecular biology, and antifungal susceptibility testing was performed using the European Committee on Antimicrobial Susceptibility Testing protocol. The ERG11 gene was sequenced to identify resistance mechanisms, and strain typing was carried out by microsatellite analysis. Results: We examined the susceptibility profile of 1315 C. parapsilosis isolates available at our reference laboratory between 2000 and 2021, noticing an increase in the number of isolates with acquired resistance to fluconazole, and voriconazole has increased in at least 8 different Spanish hospitals in 2020-2021. From 121 recorded clones, 3 were identified as the most prevalent in Spain (clone 10 in Catalonia and clone 96 in Castilla-Leon and Madrid, whereas clone 67 was found in 2 geographically unrelated regions, Cantabria and the Balearic Islands). Conclusions: Our data suggest that concurrently with the coronavirus disease 2019 pandemic, a selection of fluconazole-resistant C. parapsilosis isolates has occurred in Spain, and the expansion of specific clones has been noted across centers. Further research is needed to determine the factors that underlie the successful expansion of these clones and their potential genetic relatedness.

[Infectious disease assessment in solid organ transplant candidates]. / Evaluación de las enfermedades infecciosas en el candidato a un trasplante de órgano sólido.

BACKGROUND: Infections are one of the leading causes of morbidity and mortality in solid organ transplant recipients. Many of these infections can be prevented or their effects reduced by accurate preoperative evaluation of risk in the transplantation candidate. The elaboration of guidelines using a multidisciplinary approach can help to establish more rational diagnostic, therapeutic, and preventive measures in this setting. OBJECTIVE: To elaborate guidelines for the assessment of infectious diseases in transplant candidates, based on consensus among professionals in this field and under the auspices of Spanish scientific societies. MATERIAL AND METHODS: The Infections in Transplant Patients Group (GESITRA), within the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), appointed a panel of four microbiologists and infectious disease specialists to elaborate a draft of the guidelines, which was subsequently approved by all the members of this Group. With the support of the National Transplant Organization, the GESITRA document was then presented to various professionals in this field so they could provide their comments and suggestions. RESULTS: The final document, after incorporation of all appropriate modifications and suggestions, is presented herein. The guidelines focus on the following: a) diagnosis of active and latent infections, and identification of risk factors in the candidate; b) recommended approach for infections diagnosed during the evaluation process and their corresponding treatment; c) definition of infections contraindicating transplantation; and d) prevention of post-transplantation infectious complications by systematic vaccination and instruction on preventive measures provided to patients, their relatives, and persons living with them. DISCUSSION: Using a multidisciplinary approach that included the efforts of experts in the field and the collaboration of scientific societies, a comprehensive document containing specific recommendations was elaborated. Systematic review of the guidelines in the future is considered worthwhile by both the authors and supporters of this document.

Evaluación de las enfermedades infecciosas en el candidato a un trasplante de órgano sólido / Infectious disease assessment in solid organ transplant candidates

FUNDAMENTO. La infección es una causa mayor de morbilidad y mortalidad en el trasplantado de órgano sólido. Muchas de estas infecciones se pueden prevenir, o aminorar sus efectos, mediante la correcta evaluación del candidato en el período pretrasplante. La formulación de recomendaciones de consenso puede ayudar a establecer medidas diagnósticas, preventivas y terapéuticas más racionales. OBJETIVO. Elaborar unas guías sobre la evaluación infecciosas de los candidatos a un trasplante consensuadas por profesionales diversos y auspiciadas por sociedades científicas españolas. MATERIAL Y MÉTODOS. Un grupo de cuatro profesionales, microbiólogos e infectólogos, elaboran un borrador por encargo del Grupo de Infecciones en Trasplantados (GESITRA) de la SEIMC, que es aprobado tras discusión interna entre todos los miembros de ese Grupo. El documento de GESITRA es presentado, a través de la Organización Nacional de Trasplantes, a los diferentes profesionales relacionados con esta actividad para que aporten sus sugerencias. RESULTADOS. Se presenta el documento final que incluye todas las modificaciones y sugerencias aportadas durante el proceso de elaboración arriba mencionado. Este documento se centra en varios apartados: a) diagnóstico de las infecciones activas y latentes e identificación de los factores de riesgo en el candidato; b) la actitud a adoptar ante las infecciones diagnosticadas durante la evaluación pretrasplante y su oportuno tratamiento; c) describir las infecciones que contraindican el trasplante, y d) prevenir la aparición de complicaciones infecciosas postrasplante mediante un programa de vacunaciones y la educación sanitaria de pacientes, familiares y personas que convivirán con él. DISCUSIÓN. El proceso antes citado ha permitido elaborar un documento amplio con un enfoque multidisciplinario, que formula recomendaciones concretas. Se reconoce la conveniencia de ser revisado sistemáticamente en el transcurso del tiempo (AU)